Roche - qPCR Applications

We offer qPCR service based on latest technology with the Roche Lightcycler© LC480 for 384 well plates and a Qiagen BioRobot for automated plate setup, permitting high throughput qPCR studies for array validation, or as stand-alone experiments. We offer the entire range of qPCR applications to meet your demands. You will benefit from our long-standing experience in qPCR.

• Assay Design and Evaluation
Depending on your needs, experimental questions and study size, we will advise you on the choice of the detection formats. We will also design primers and probes for you. Each assay is optimized and evaluated to obtain optimal specificity and sensitivity.

• Expression Profiling (Relative Quantification of mRNA)
RT-qPCR is considered the gold standard for gene expression studies. After assay development, we will measure raw Cp values for your target genes and a choice of appropriate reference genes. After evaluation of the reference genes, we will calculate normalized expression values.

• Absolute Quantification
Using known standards, a calibration curve is generated to determine absolute copy numbers of your target gene in your sample.

• DNA Copy Number Calculation
Following the same principles as for calculation of relative expression, we apply qPCR to determine gains and losses in genomic DNA compared to a blend of standard genomic DNAs (human) or any reference DNA suitable for your samples.

• Gene Scanning/High-Resolution Melting Analysis
High resolution melting (HRM) analysis is a fast and inexpensive method for genotyping known SNPs or identifying new polymorphisms. DNA methylation status of CpG sites can be determined in bisulphate-treated DNA.

• SNP Genotyping
With the widely used TaqMan® probes, the genotypes of any known SNP can easily be identified.

Roche 454

The Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry, is the flagship 454 Sequencing platform. Offering more than 1 million high-quality reads per run and read lengths of 400 bases, the system is ideally suited for de novo sequencing of whole genomes and transcriptomes of any size, metagenomic characterization of complex samples, resequencing studies and more. The GS FLX System is at the heart of breakthrough scientific discoveries and hundreds of peer-reviewed publications to date.